Lung injury produced by moderate lung overinflation in rats.
نویسندگان
چکیده
In models using both intact animals and isolated lungs of rats, rabbits, lambs, and dogs, application of high airway pressure and resultant lung overdistension has been shown to cause lung injury. Under pathological conditions, the more normal portions of the lung may be subjected to relative overdistension when supranormal airway pressures are used. A small animal model of lung overdistension is presented here to demonstrate that lung injury can be caused by moderate lung overdistension. Rats were anesthetized with Ketamine and pentobarbital. A tracheostomy was performed and arterial and central venous catheters placed. The animal was placed in a plethysmograph and an infant ventilator was used to provide mechanical ventilation. In the first experiment, animals in group A (n = 6, control) were ventilated at tidal volume (TV) of 5 mL/kg for 5 hours. Animals in group B (n = 6) were ventilated at TV 15 mL/kg for 60 minutes and then were returned TV of 5 mL/kg for the remainder of the experiment. Animals in group C (n = 5) were ventilated at TV 20 mL/kg for 60 minutes and then returned to TV 5 mL/kg. Static lung compliance and arterial blood gasses were measured. Wet and dry lung weight were obtained after sacrifice. In the second experiment, animals in group D (n = 4, control) were ventilated at TV 5 mL/kg for 240 minutes and killed. Animals in group E (n = 4) were ventilated at TV 20 mL/kg for 60 minutes, returned to TV 5 mL/kg, and killed at 240 minutes. Fluorescein-labeled albumin was administered to each animal prior to mechanical ventilation.(ABSTRACT TRUNCATED AT 250 WORDS)
منابع مشابه
Protective effect of S-nitrosoglutathione pretreatment on acute lung injury in septic rats
Objective(s): To investigate the protective effect of S-nitrosoglutathione (SNG) pretreatment on acute lung injury (ALI) in septic rats. Materials and Methods: We constructed a rat model of sepsis by cecal ligation and perforation (CLP), and randomly divided into Sham, CLP, and CLP+SNG (0.25 and 0.5 mg/kg) groups. We used H&E; staining an...
متن کاملProtective effect of vitamin D on radiation-induced lung injury: Experimental evidence
Background: Vitamin D, especially its most active metabolite 1,25-dihydroxyvitamin D₃(Vit D) is essential in regulating a wide variety of biologic processes, such as regulating mesangial cell activation. The objective of this study was to assess the histopathological changes of effectiveness of Vit D as a protective agent against radiation induced lung injury. Materials and Methods: Eighteen Wi...
متن کاملEstablishment of a rat model of radiation-induced lung injury
Background: Radiation-induced lung injury is a refractory side effect in lung cancer radiotherapy, the mechanism still remains unclear, hence an appropriate animal mode may become useful to investigate it. Materials and Methods: 50 female Wistar rats were randomly divided into 5 groups, average 10 rats/cage: A. control group B. 3Gy×10f C. 6Gy×5f D. 12.5Gy×1f E.15.3Gy×1f....
متن کاملRetracted: Time course changes of oxidative stress and inflammation in hyperoxia-induced acute lung injury in rats
متن کامل
Asiaticoside attenuates hyperoxia-induced lung injury in vitro andin vivo
Objective(s): Asiaticoside (AS) displays anti-inflammation, and anti-apoptosis effect, but the role of AS in hyperoxia-induced lung injury (HILI) treatment is undefined. Therefore, the aim of this study was to investigate the effects of AS on HILI on premature rats and alveolar type II (AEC II) cells.Materials and Methods: Sprague-Dawley...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of pediatric surgery
دوره 28 3 شماره
صفحات -
تاریخ انتشار 1993